Dudley and an Unnamed Rabbit Cop once referred to the Chameleon as the "Cham-ah-lee-on", which the Chameleon himself also mispronounces later once.
He is the villain who is picked-on most for his near awkward and obsessive behavior. He is willing to be friends with characters on both sides of the spectrum; D.O.O.M. and T.U.F.F. both, and neither one of them seem to enjoy his presence.
The whole moving eyes thing is an attribute of real chameleons.
Every time he is seen working with another villain, they take advantage of him. In the episode Mission: Really Big Mission, Snaptrap uses him as a cupholder, box of tissues, and a toothpick. The Chameleon of course doesn't seem to mind because he feels like he is "helping."
When the Chameleon is shape-shifting, he is all bionic like his suit and his eyes are red and straight.
A running gag is that every time he is seen, he wears some kind of eyewear that usually either breaks or that doesn't usually fit over his eyes.
Sometimes, he changes his voice based on the audience's knowledge of what's going on the show. One example is in the episode "Share-A-Lair". He changes his voice when he turns into Agent Rodentski, but he doesn't change his voice when he turns back into Agent Rodentski later on in the episode.
We've seen the Chameleon three times without the molecular transformation suit, and that's in "Puppy Love" (prison uniform), "Guard Dog" (his underwear), and "Diary of a Mad Cat" (a hat, sunglasses, a purple shirt and red swimming shorts). It's possibly rare for the Chameleon to be seen without his Molecular Transformation Suit, but three times the charm.
In some episodes, his eyes shrink with the irises smaller and lacking pupils.
It seems he isn't fond of children, revealed in "Snap Dad," as he admitted to Peg that he didn't know she had kids and soon ran away from her, afterward, for this reason.
In Hot Dog, The Chameleon doesn't like wearing winter clothes because they're not "Diabolical".
In the German dub of T.U.F.F. Puppy, The Chameleon's voice is higher pitched and cracking. In the Spanish dub, his voice almost sounds monotonous.
In A Doomed Christmas he can be seen disguised as a Christmas tree in his current jail cell.
It's revealed in Guard Dog that The Chameleon was a unsuccessful author and created a story called "The Very Lonely Lizard". In the same episode, The Chameleon wears headgear and a mud mask when going to bed due to his Chronic Eczema and underbite.
It was revealed in A Doomed Christmas that The Chameleon has a sister named Camille. In the same episode, the Chameleon enjoys Christmas despite being a villain.
The opening, online game, and promotional picture showed that the Chameleon was originally going to be a member of D.O.O.M.. It is unknown why the creators decided to remove him from D.O.O.M. Another reason why they have done this is that, like in "Share-A-Lair", that he has no interest in them.
The only episode he does not transform in is Snap Dad, as he only appeared in the last few seconds of the episode.
In Dog Tired, he's the host of the Evil Shopping Channel.