The episode's title is a play on the idiom "toast of the town", which refers to someone who is most admired in a particular place. Keswick's referral to R.I.T.A. as the "toast of T.U.F.F." is a double entendre: not only ironic because Kitty and Dudley despise her, but because her primary directive is to toast food.
This is the first time that T.U.F.F.'s name is used in the title of an episode.
R.I.T.A. taking over the T.U.F.F. HQ's electronic mainframe is similar to H.A.L. taking over the ship in 2001: A Space Odyssey.
Several of Keswick's physical characteristics are described: A "pouch", gills, webbed feet, and egg-laying abilities.
This was the first episode where a near-death experience has occurred.
Kitty mentioned that she spent eight years in spy college, which might be a hint of her age.
This is the first and only known episode where The Chief gains weight.
This is the first time that Dudley gains weight. The second time is in Cold Fish, and the third is in Barking Bad.
This is the first episode to not have fighting.
The background design of the 4th dimension looks like the background of the GhostZone from Butch Hartman's other show Danny Phantom.
Keswick said the title of the episode in one scene.
Someone mistaking one of Keswick's holograms for the real Keswick and hologram Keswick says that he is "over there".
When The Chameleon is turned into a worm, the Molecular Transformation Suit disappears!
At D.O.O.M. HQ it is night, but at the Chameleon's house, it is daytime. It is possible that it was midnight when they were at the D.O.O.M. HQ, and turned into morning when they went to The Chameleon's house.
Keswick said that he sent Billy into the 4th dimension; technically the 4th dimension is time-travel. so Billy should have been sent in time instead of another dimension.